An improved method for nanogoldin situhybridization visualized with environmental scanning electron microscopy

An Improved Staining Method for Electron Microscopy

A n a q u e o u s so lu t ion of u r a n y l ace t a t e was first e m p l o y e d as a n e l ec t ron s ta in by W a t s o n (1958). W e h a v e f o u n d t h a t this s ta in , a l t h o u g h f r e q u e n t l y effective, is capr ic ious . T h e s ta in m a y no t inc rease con t r a s t of a g iven spec imen , a n d m a y , in o the r ins tances , l eave u n d e s i r a b l e c o n t a m i...

Bibliography of environmental scanning electron microscopy.

Two updated lists of publications on environmental scanning electron microscopy are compiled. One list contains mainly those papers dealing with the development and instrumentation, while the other deals mainly with the applications of the technique. A brief introductory summary of the field is presented.

Environmental scanning electron microscopy for biology and polymer science

Electron microscopy of materials in a gaseous environment has its origins as far back as about the 1950s for the transmission electron microscope (TEM), later followed by developments for the scanning electron microscope (SEM). Focusing on the latter, this review outlines the history and commercial development of the technology, culminating in the environmental SEM (ESEM), and the techniques fo...

Improved method for sequential study of an isolated glomerulus by scanning electron, transmission electron, and light microscopy.

Live Bacterial Physiology Visualized with 5 nm Resolution Using Scanning Transmission Electron Microscopy.

It is now possible to visualize at nanometer resolution the infection of a living biological cell with virus without compromising cell viability using scanning transmission electron microscopy (STEM). To provide contrast while preserving viability, Escherichia coli and P1 bacteriophages were first positively stained with a very low concentration of uranyl acetate in minimal phosphate medium and...